CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated) technology revolutionized the field of genetic engineering with its ability to edit DNA with remarkable precision. This technology has found vast applications in numerous scientific disciplines, ranging from agricultural biotechnology to therapeutic development, and has proven instrumental in solving complex genetic challenges.
Recognizing the unmet needs of the scientific community, Amerigo Scientific offers a comprehensive collection of CRISPR/Cas enzymes from global manufacturers covering all the core biological research areas. These effective enzymes promise to unlock an array of previously unattainable possibilities and drive pioneering breakthroughs in genetic research.
Among these CRISPR/Cas enzymes, SpCas9 nuclease is a DNA endonuclease guided by crRNA and tracrRNA (or sgRNA alone), which is derived from S. pyogenes. SpCas9 specifically recognizes and cleaves dsDNA targets in a PAM (NGG)-dependent manner. The cleavage site of Cas9 in the target sequence is 3 bps away from the PAM site. In addition, studies have found that SpCas9 can specifically cleave ssDNA or ssRNA in the presence of DNA PAMmer sequences and can also be used in in vitro experiments for cleaving target DNA and cloning target fragments.
Featuring an assortment of highly configurable enzymes, Amerigo Scientific's CRISPR/Cas enzymes empower researchers to precisely target specific genes, facilitating more accurate manipulation and modification of genetic material. The enzymes’ unique characteristics make them particularly valuable for applications such as knockout studies, gene therapy development, precision agriculture, and countless other genetic engineering endeavors.
about Amerigo Scientific
Amerigo Scientific, as a recognized distributor in the United States, collaborates closely with leading manufacturers worldwide and invites cooperation to all companies and institutions in the branch of reagents, kits, antibodies, and many other products for life science, biochemistry, and biotechnology. Its professional team is equipped with excellent technical support and thoughtful customer service. As most of its employees have earned a graduate (Ph.D. or M.S.) degree in life science, they can comprehend customers’ questions or concerns and are always ready to provide individualized customer service of high standards.
